Investigations were carried out in the course of establishing an in vitro germplasm bank to determine the response of a number of genotypes to shoot tip culture and various low temperature (6C) culture storage treatments. Average multiplication rates in shoot tip culture in R. nigrum cultivars ranged from 1.5 in Seabrooks Black to 3.5% in Ben Lomond. Survival following cold storage for 3 weeks was 95-100%. For each genotype tested multiplication rates in at least one treatment were better than in the control.