RAPD markers were generated from 13 different Rubus species in order to assess the degree of similarity between species from the important subgenera Idaeobats, Eubats and Anoplobats. All ten primers revealed scorable polymorphisms within both the closely related and the genetically diverse individuals. Some 372 markers were generated and scored from the material analysed. Estimates of similarity, dendrograms and principle coordinate analysis were calculated, with the results generally being in agreement with previous classifications of the species studied, confirming the validity and usefulness of the RAPD method. However, amongst the species studied, R. macraei (subg. Idaeobats) proved more diverse and grouped in with both the subgenera Idaeobats and Eubats at only 26% similarity.