Various media and sources of explant of raspberry cultivars Autumn Bliss and SCRI 8242E6, blackberry X raspberry cultivars Tayberry, Tummelberry and Sunberry, and the blackberry cv. Loch Ness were assessed for their ability to regenerate whole plants in vitro. Regenerants were produced from leaf discs and from both peeled and unpeeled internodal stem segments but not from epidermal peelings. Factors crucial to plantlet regeneration were hormone type and concentration, amount of sucrose, absence of activated charcoal, presence of light, and for leaf discs their orientation with adaxial surface uppermost. Genotypes differed considerably in their capacity to regenerate.