The isolation of polymorphic codominant microsatellite markers in Rubus, red raspberry (cv. Glen Moy) in particular, will provide a tool to investigate gene flow between cultivated and wild raspberries. Microsatellite loci were isolated by screening a PstI size selected genomic library with AC(13) and AG(13). Positive clones were sequenced and primer pairs designed to the sequences flanking identified simple sequence repeats. One primer of each pair was fluorescently labelled to facilitate polymerase chain reaction (PCR) product identification on an automated DNA sequencer. We describe 10 polymorphic microsatellite loci developed and demonstrate their usefulness in different Rubus species.